ABSTRACT
Objective:To establish the purification process of citral in volatile oil from Fructus Litseae by molecular distillation . Methods:The twice molecular distillation , GC and area normalization method were used for extracting citral , detecting the contents and describing the efficacy of purification , respectively .The mainly factor concerned was temperature .The conditions were as follows:the system pressure was 3000 Pa, the scraper speed was 300 r· min-1 , the feeding rate was 7.5 ml· min-1 and the distillation tem-perature was 45℃for the first time molecular distillation; the system pressure was 5 Pa, the scraper speed was 300 r· min-1 , the feeding rate was 7.5 ml· min-1 and the distillation temperature was 45℃ for the second time molecular distillation .GC was utilized under the following conditions:the sample injection was 1 μl, the column temperature was programmed from 70℃to 250℃, the split ratio was 1:100 and the flow rate of carrier gas was 1.0 ml· min-1 .Results: The content and yield of citral was up to 95.0％ and 87.5％, respectively.Conclusion:The method of twice molecular distillation in combination with GC to purify and detect citral is es -tablished in the work, which can provide reference for the research on the chemical components of Litsea cubeba(Lour.) Pers.and the preparation of citral .
ABSTRACT
Investigatetion of the fruits and leaves oil of Litsea cubeba (Lour.) Pers. wild growing in the moutain areas in Ba Vi district, Ha Tay province. The major components of the fruits oil of Litsea cubeba (Lour.) Pers. is citral, which includes of neral (citral b) and geranial (citral a) with content over 65% equivalent in some Northern provinces. Fruits and leaves oil analysis were carried out by a combination of capilarry GC and MS. The main components of the leaves oil are linalol (over 85%), 1.8-cineol (-50%), sabinen (10-15%) and -terpineol or -terpinyl acetate (10-20%). This plant in Ba Vi has many biochemical diversification, that is also a character of Lauraceae as Long nao, Vu huong.